| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 2 | Laury MILETTE | Team Abadie Magnan | 1:06:31. | 1:06:31 |
| 2 | 209 | Nadiya LINDE-ELMHIRST | Milton Revolution Racing | 1:06:32. | M.T. |
| 3 | 9 | Kaitlyn RAUWERDA | Cyclery Racing - Abacus Data | 1:06:33. | M.T. |
| 4 | 12 | Annie SCOTT | Cyclery Racing - Abacus Data | 1:06:49. | 18 |
| 5 | 212 | Eva GABELIER | Equipe du Quebec | 1:06:49. | M.T. |
| 6 | 27 | Claire ABREY | Team Winston Salem | 1:06:50. | M.T. |
| 7 | 42 | Emilie FORTIN | Minimax Cycling Team | 1:06:53. | 22 |
| 8 | 15 | Katja VERKERK | Minimax Cycling Team | 1:07:15. | 44 |
| 9 | 8 | Camille DESROCHERS LAFLAMME | SpeedBlock-Terun | 1:07:16. | M.T. |
| 10 | 28 | Maggie COLES-LYSTER | Human Powered Health | 1:07:16. | M.T. |
| 11 | 218 | Abigael FORTIER | TaG Cycling Race Team | 1:07:16. | M.T. |
| 12 | 1 | Alison JACKSON | St Michel - Preference Home - Auber93 | 1:07:17. | M.T. |
| 13 | 215 | Clemence COUSINEAU | KDM Pack Cycling | 1:07:17. | M.T. |
| 14 | 213 | Amaia ERVIN-ARAMBARRI | TaG Cycling Race Team | 1:07:17. | M.T. |
| 15 | 16 | Noemie BEAULIEU | | 1:07:17. | M.T. |
| 16 | 41 | Jazmine LAVERGNE | Team Abadie Magnan | 1:07:17. | M.T. |
| 17 | 10 | Mairen LAWSON | Aegis X Leaders of Enchantment | 1:07:18. | M.T. |
| 18 | 219 | Eleanor WINCHELL | Milton Revolution Racing | 1:07:19. | M.T. |
| 19 | 207 | Addison FRANK | UVCA Troyes | 1:07:19. | M.T. |
| 20 | 206 | Dylan BAKER | Cyclery Racing - Abacus Data | 1:07:20. | M.T. |
| 21 | 17 | Skyler GOUDSWAARD | Cyclery Racing - Abacus Data | 1:07:20. | M.T. |
| 22 | 201 | Alexandra VOLSTAD | EF Education-Oatly | 1:07:20. | M.T. |
| 23 | 204 | Mathilde HUOT | Team Abadie Magnan | 1:07:20. | M.T. |
| 24 | 31 | Sarina DAIGLE | Passion Project | 1:07:21. | M.T. |
| 25 | 23 | Elisa LUO | Monarch Racing | 1:07:21. | M.T. |
| 26 | 205 | Coralie HOUDE | Equipe du Quebec | 1:07:22. | M.T. |
| 27 | 22 | Allyson WEBB CHARLAND | Dynamiks | 1:07:23. | 52 |
| 28 | 33 | Olivia RUBENS | Cyclery Racing - Abacus Data | 1:07:23. | M.T. |
| 29 | 21 | Audrey RIECKENBERG | United Nations of Cyclists (UNC) | 1:07:24. | M.T. |
| 30 | 216 | Raphaelle HOUDE | Equipe du Quebec | 1:07:25. | M.T. |
| 31 | 224 | Julianne RICHARD | NORD Devo | 1:07:26. | M.T. |
| 32 | 223 | Florence FILLION | Olympia CC | 1:07:26. | M.T. |
| 33 | 211 | Vanessa MONTRICHARD | TaG Cycling Race Team | 1:07:26. | M.T. |
| 34 | 225 | Naomie JULIEN | WV Schijndel | 1:07:26. | M.T. |
| 35 | 208 | Taylor TOMPKINS | Milton Revolution Racing | 1:07:31. | 1:00 |
| 36 | 229 | Kristen TAYLOR | Cyclery Racing - Abacus Data | 1:07:35. | 1:04 |
| 37 | 39 | Elizabeth SMITH | Dynamiks | 1:07:36. | M.T. |
| 38 | 36 | Evelyne GAGNON | Dynamiks | 1:07:56. | 1:25 |
| 39 | 234 | Mia DE MARTIN | Dynamiks | 1:08:23. | 1:52 |
| DNF | 18 | Daphnee BEAUCHAMP | Dynamiks | | |
| DNF | 24 | Amelie GAUTHIER | Normandin Musette EC | | |
| DNF | 30 | Raylan STROUD | Cyclery Racing - Abacus Data | | |
| DNF | 210 | Cadie GEERTSEMA | Cyclery Racing - Abacus Data | | |
| DNF | 220 | Marianne BEAUMIER | NORD Devo | | |
| DNF | 226 | Justine MERCIER | Dynamiks | | |
| DNF | 227 | Dorothee PERRON | Passion Project | | |
| DNF | 228 | Corinne POULIOT | Normandin Musette EC | | |
| DNF | 231 | Ashton THOMSON | Milton Revolution Racing | | |
| DNF | 233 | Evaelle FORTIER | Dynamiks | | |
| DNS | 14 | Laetitia BILODEAU | Passion Project | | |
| DNS | 32 | Charlotte MITCHELL | Analog Racing Team | | |
| DNS | 35 | Sara EVERSON | Cyclery Racing - Abacus Data | | |
| DNS | 214 | Lea GAUTHIER | Dynamiks | | |
| DNS | 217 | Aislin HALLAHAN | Milton Revolution Racing | | |
