| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 1 | Lieke NOOIJEN | TEAM VISMA LEASE A BIKE | 1:36:39. | 1:36:39 |
| 2 | 5 | Nienke VEENHOVEN | TEAM VISMA LEASE A BIKE | 1:36:40. | M.T. |
| 3 | 34 | Sofia ARREOLA NAVARRO | VIRGINIAS BLUE RIDGE TWENTY28 | 1:36:40. | M.T. |
| 4 | 54 | Skyler GOUDSWAARD | CYCLERY RACING | 1:36:40. | M.T. |
| 5 | 74 | Mathilde HUOT | MILTON REVOLUTION RACING | 1:36:40. | M.T. |
| 6 | 6 | Sophie VON BERSWORDT-WALLRABE | TEAM VISMA LEASE A BIKE | 1:36:40. | M.T. |
| 7 | 103 | Josephine PELOQUIN | LA SWEAT | 1:36:40. | M.T. |
| 8 | 112 | Karlijn KOOPS | ORION RACING | 1:36:41. | M.T. |
| 9 | 2 | Carlijn ACHTEREEKTE | TEAM VISMA LEASE A BIKE | 1:36:41. | M.T. |
| 10 | 4 | Margaux VIGIE | TEAM VISMA LEASE A BIKE | 1:36:41. | M.T. |
| 11 | 3 | Martina FIDANZA | TEAM VISMA LEASE A BIKE | 1:36:41. | M.T. |
| 12 | 41 | Laury MILETTE | EQUIPE DU QUEBEC | 1:36:41. | M.T. |
| 13 | 91 | Katherine RUSCH | CCB P/B LEVINE LAW GROUP | 1:37:23. | 44 |
| 14 | 21 | Lucie FITYUS | AUSTRALIE | 1:37:23. | M.T. |
| 15 | 123 | Stephanie HALAMEK | UNITED CYCLING PB CADENCE CYCLERY | 1:37:24. | M.T. |
| 16 | 15 | Dylan BAKER | CANADA | 1:37:24. | M.T. |
| 17 | 122 | Florence HOWDEN | UNITED CYCLING PB CADENCE CYCLERY | 1:37:24. | M.T. |
| 18 | 14 | Amaia ERVIN-ARAMBARRI | CANADA | 1:37:24. | M.T. |
| 19 | 23 | Sophie MARR | AUSTRALIE | 1:37:24. | M.T. |
| 20 | 42 | Camille PRIMEAU | EQUIPE DU QUEBEC | 1:37:24. | M.T. |
| 21 | 24 | Sophia SAMMONS | AUSTRALIE | 1:37:25. | M.T. |
| 22 | 62 | Elizabeth SMITH | DYNAMIKS | 1:37:25. | M.T. |
| 23 | 16 | Adele NORMAND | CANADA | 1:37:25. | M.T. |
| 24 | 13 | Coralie HOUDE | CANADA | 1:37:25. | M.T. |
| 25 | 154 | Jamie CHAPMAN | RIDE WITH RENDALL P/B BIEMME | 1:37:25. | M.T. |
| 26 | 104 | Marjie BEMIS | LA SWEAT | 1:37:26. | M.T. |
| 27 | 92 | Ella BRENNEMAN | CCB P/B LEVINE LAW GROUP | 1:37:26. | M.T. |
| 28 | 151 | Emily FLYNN | RIDE WITH RENDALL P/B BIEMME | 1:37:27. | 48 |
| 29 | 35 | Emily GILBERT | VIRGINIAS BLUE RIDGE TWENTY28 | 1:37:28. | M.T. |
| 30 | 12 | Julie LACOURCIERE | CANADA | 1:37:28. | M.T. |
| 31 | 133 | Emily NEILL | ANALOG RACING TEAM | 1:37:28. | M.T. |
| 32 | 44 | Iris GABELIER | EQUIPE DU QUEBEC | 1:37:28. | M.T. |
| 33 | 45 | Laetitia BILODEAU | EQUIPE DU QUEBEC | 1:37:28. | M.T. |
| 34 | 22 | Odette LYNCH | AUSTRALIE | 1:37:28. | M.T. |
| 35 | 51 | Annie SCOTT | CYCLERY RACING | 1:37:29. | M.T. |
| 36 | 121 | Kathryn AMAN | UNITED CYCLING PB CADENCE CYCLERY | 1:37:29. | M.T. |
| 37 | 126 | Betty HASSE | UNITED CYCLING PB CADENCE CYCLERY | 1:37:29. | M.T. |
| 38 | 84 | Micha GUTMANIS | RED TRUCK RACING PB MOSAIC HOMES | 1:40:10. | 3:31 |
| 39 | 93 | Sabrina HAYES | CCB P/B LEVINE LAW GROUP | 1:40:10. | M.T. |
| 40 | 102 | Holly SIMONSON | LA SWEAT | 1:40:10. | M.T. |
| 41 | 55 | Raylan STROUD | CYCLERY RACING | 1:40:11. | M.T. |
| 42 | 105 | Regina DOTY | LA SWEAT | 1:40:11. | M.T. |
| 43 | 33 | Olivia CUMMINS | VIRGINIAS BLUE RIDGE TWENTY28 | 1:40:11. | M.T. |
| 44 | 32 | Kenna PFEIFFER | VIRGINIAS BLUE RIDGE TWENTY28 | 1:40:11. | M.T. |
| 45 | 43 | Camille DESROCHERS LAFLAMME | EQUIPE DU QUEBEC | 1:40:12. | M.T. |
| 46 | 144 | Elissa PROULX | ECF P/P GOLDEN SPORT | 1:40:12. | M.T. |
| 47 | 63 | Lea GAUTHIER | DYNAMIKS | 1:40:12. | M.T. |
| 48 | 85 | Yasmin JACKSON | RED TRUCK RACING PB MOSAIC HOMES | 1:40:12. | M.T. |
| 49 | 111 | Sara YOUMANS | ORION RACING | 1:40:12. | M.T. |
| 50 | 132 | Eleanor ROBERTS | ANALOG RACING TEAM | 1:40:12. | M.T. |
| 51 | 56 | Whitney SURGENOR | CYCLERY RACING | 1:40:13. | M.T. |
| 52 | 73 | Nadiya LINDE-ELMHIRST | MILTON REVOLUTION RACING | 1:40:13. | M.T. |
| 53 | 72 | Skye PELLERIN | MILTON REVOLUTION RACING | 1:40:13. | M.T. |
| 54 | 53 | Emily DRIEDGER | CYCLERY RACING | 1:40:13. | M.T. |
| 55 | 146 | Catherine SANTERRE | ECF P/P GOLDEN SPORT | 1:40:14. | M.T. |
| 56 | 153 | Nina VARAH | RIDE WITH RENDALL P/B BIEMME | 1:40:14. | M.T. |
| 57 | 66 | Eva GABELIER | DYNAMIKS | 1:40:14. | M.T. |
| 58 | 94 | Elizabeth GRIER | CCB P/B LEVINE LAW GROUP | 1:40:14. | M.T. |
| 59 | 86 | Vanessa MONTRICHARD | RED TRUCK RACING PB MOSAIC HOMES | 1:40:14. | M.T. |
| 60 | 101 | Ngaire BARRACLOUGH | LA SWEAT | 1:40:15. | M.T. |
| 61 | 82 | Cynthia PHAM | RED TRUCK RACING PB MOSAIC HOMES | 1:40:15. | M.T. |
| 62 | 71 | Taylor TOMPKINS | MILTON REVOLUTION RACING | 1:40:15. | M.T. |
| 63 | 11 | Mairen LAWSON | CANADA | 1:40:15. | M.T. |
| 64 | 152 | Zoe ADAM | RIDE WITH RENDALL P/B BIEMME | 1:40:16. | M.T. |
| 65 | 125 | Tatum BROWN | UNITED CYCLING PB CADENCE CYCLERY | 1:40:16. | M.T. |
| 66 | 145 | Jasmine PAQUETTE | ECF P/P GOLDEN SPORT | 1:40:16. | M.T. |
| 67 | 81 | Brenna PAULY | RED TRUCK RACING PB MOSAIC HOMES | 1:44:12. | 7:33 |
| 68 | 124 | Jennifer THORNTON-BROOKS | UNITED CYCLING PB CADENCE CYCLERY | 1:45:05. | 8:26 |
| 69 | 76 | Ashton THOMSON | MILTON REVOLUTION RACING | 1:46:27. | 9:48 |
| 70 | 155 | Olivia RUBENS | RIDE WITH RENDALL P/B BIEMME | 1:46:27. | M.T. |
| 71 | 115 | Alison MERNER | ORION RACING | 1:46:27. | M.T. |
| 72 | 64 | Justine MERCIER | DYNAMIKS | 1:46:28. | M.T. |
| 73 | 113 | Kara LILLY | ORION RACING | 1:46:28. | M.T. |
| 74 | 52 | Sara EVERSON | CYCLERY RACING | 1:46:28. | M.T. |
| 75 | 65 | Allyson WEBB CHARLAND | DYNAMIKS | 1:46:29. | M.T. |
| 76 | 83 | Kathryn AYROUD | RED TRUCK RACING PB MOSAIC HOMES | 1:46:29. | M.T. |
| 77 | 75 | Emilie MCINTYRE | MILTON REVOLUTION RACING | 1:46:29. | M.T. |
| 78 | 156 | Elizabeth ARCHBOLD | RIDE WITH RENDALL P/B BIEMME | 1:46:30. | M.T. |
| 79 | 134 | Dahlia ROHM | ANALOG RACING TEAM | 1:46:33. | 9:54 |
| 80 | 136 | Ashley MCMANUS | ANALOG RACING TEAM | 1:46:33. | M.T. |
| DNF | 31 | Emily EHRLICH | VIRGINIAS BLUE RIDGE TWENTY28 | | |
| DNF | 46 | Megan POULIN | EQUIPE DU QUEBEC | | |
| DNF | 61 | Evaelle FORTIER | DYNAMIKS | | |
| DNF | 114 | Julissa Andrea ALVARADO JARAMILLO | ORION RACING | | |
| DNF | 131 | Ava HACHMANN | ANALOG RACING TEAM | | |
| DNF | 135 | Elizabeth VAAGS | ANALOG RACING TEAM | | |
| DNF | 141 | Isabelle MAYRAND | ECF P/P GOLDEN SPORT | | |
| DNF | 142 | Maude MC ALLISTER | ECF P/P GOLDEN SPORT | | |
| DNF | 143 | Naomi BLOUIN | ECF P/P GOLDEN SPORT | | |
